Oxion Inverso

Highlights

  • Life Science applications
  • Brightfield, phase contrast and fluorescence
  • Revolving quintuple nosepiece
  • 100 W mercury vapor lamp for fluorescence
  • Observation of tissue cultures, living cells
  • Long working distance IOS plan achromatic objectives
  • 5 W LED transmitted illumination

Models

Oxion Inverso

MODELS LWD Plan 10x/20x/40x objectives LWD Plan phase 10x/20x/40x objectives LWD Plan Fluarex 10x/20x/40x objectives LWD Plan Fluarex 10x and plan phase fluarex 20x/40x objectives 2-position filter block 4-position filter block Mechanical stage  
OX.2003‑PL
OX.2053‑PL
OX.2003‑PLPH
OX.2053‑PLPH
OX.2253‑PLF  
OX.2453‑PLF  
OX.2253‑PLPHF
OX.2453‑PLPHF

Fluarex objectives are made of low-absorption glass for fluorescence applications
Fluarex is a trademark of Euromex microscopen bv
LWD = long working distance


Specifications

EYEPIECES
Pair of DIN HWF plan 10x/22 mm eyepieces

HEADS
• Trinocular 45° inclined tubes with extended low and high positions for eyepieces
• One diopter adjustment on left eyepiece
• Interpupillary distance of 54 to 75 mm
• Trinocular head with 100-0 % and 80-20 % positions

NOSEPIECES
Revolving quintuple nosepiece on ball-bearings

OBJECTIVES FOR BRIGHTFIELD 
(models OX.2003-PL and OX.2053-PL)

• Infinity color corrected long working distance plan 10x/0.25, 20x/0.40, 40x/0.60 IOS objectives
• An optional 60x/0.70 IOS objective is also available

OBJECTIVES FOR PHASE CONTRAST
(models OX.2003-PLPH and OX.2053-PLPH)

Infinity color corrected long working distance plan phase 10x/0.25, 20x/0.40, 40x/0.60 IOS objectives 

OBJECTIVES FOR BRIGHTFIELD AND FLUORESCENCE MICROSCOPY
(models OX.2253-PLF and OX.2453-PLF)

• Infinity color corrected long working distance plan Fluarex 10x/0.30, 20x/0.45, 40x/0.65 IOS objectives
• An optional plan Fluarex 60x/0.75 IOS objective is also available

OBJECTIVES FOR PHASE CONTRAST AND FLUORESCENCE MICROSCOPY
(models OX.2253-PLPHF and OX.2453-PLPHF)

Infinity color corrected long working distance plan fluarex 10x/0.30 and plan phase fluarex 20x/0.45 and S40x/0.65 IOS objectives. 

All objectives corrected for 1.2 mm thickness. 
All optics are anti-fungus treated and anti-reflection coated for maximum light throughput 

STAGE
Stage 250 x 230 mm equipped with a coaxial mechanical 120 x 78 mm X-Y stage, delivered with:

• metallic insert for Ø 35 mm cell culture dish dishes
• 76 x 26 mm slide holder
• glass insert with hole

optional universal cell culture dish holder

CONDENSER
• N.A. 0.30 condenser with iris diaphragm. Working distance 72 mm
• The models for the phase contrast OX.2003-PLPH, OX.2053- PLPH, OX.2253-PLPHF and OX.2453-PLPHF are delivered with a slider with 4x/10x and 20x/40x pre-centered phase rings

FOCUSING ADJUSTMENT
Coaxial 25 mm coarse and fine adjustments, 2 μm precision and with friction adjustment

ILLUMINATION
• Transmitted 5 W LED Intensity adjustable with internal 100-240 V power supply. Fuse holder 3.15 A / 250 V. 
• The fluorescence models are equipped with a reflected 100 W HBO mercury vapor light source with external 100-240 V power supply, fuse holder 3.15 A / 250 V, UV protection shield

FILTERS FOR PHASE CONTRAST MODELS
Interference filter 550 nm green and blue filter, 45 mm diameter 

STANDARD FLUORESCENCE FILTERS
(for fluorescence models)

The fluorescence models OX.2253-PLF and OX.2453-PLF are standard supplied with two filter sets for excitation:
- in the blue spectrum, OX.9745, EX465-495 (BP) DM505 (LP) EM515-558 (BP)
- in the green spectrum, OX.9746, EX520-600 (BP) DM600 (LP) EM605-665 (BP)

The fluorescence models OX.2253-PLF and OX.2453-PLPHF equipped with a 4 positions filter rail equipped with three standard filter sets for excitation:
- in the blue spectrum, OX.9745, EX465-495 (BP) DM505 (LP) EM515-558 (BP)
- in the green spectrum, OX.9746, EX520-600 (BP) DM600(LP) EM605-665 (BP)
- in the UV spectrum, OX.9747, EX361-381 (BP) D415 (LP) EM435-495 (BP)

STANDARD GENERAL PURPOSE FLUORESCENCE FILTERS SETS

 OX.9745_1OX.9745 Blue excitation 465-495 nm

FITC EX465-495 (BP) DM505 (LP) EM515-558 (BP) 
Also suitable for: Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald GFP, FAM, FITC, Fluo-4, GFP LysoTracker Green/pH 5.2, MitoTracker Green FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen

OX.9746_1 OX.9746 Green excitation 520-600 nm

Texas Red / mCherry / Alexa Fluor 594 EX520-600 (BP) DM600 (LP) EM605-665 (BP)
Also suitable for: Alexa Fluor 568™, Alexa Fluor 594™, CAL Fluor® Red 610, Cy3.5™, DyLight 594, FusionRed, Killer Red, mCherry, MitoTracker Red/MeOH, mKate2, mRFP1, ReAsH-CCPGCC, Rhodamine Red™-X, ROX, Sulforhodamine 101, Texas Red®, Texas Red®-X, X-rhod-1/Ca2+

OX.9747_1 OX.9747 UV excitation 361-381 nm

DAPI / Hoechst / Alexa Fluor 350 EX361-381 (BP) D415 (LP) EM435-495 (BP)
Also suitable for: Alexa Fluor 350™, Biosearch Blue, Coumarin, DAPI, DyLight 350, Hoechst 33258, LysoTracker Blue/MeOH


OX.9748_1 OX.9748 Violet excitation 361-389 nm

UV / DAPI Longpass, Calcofluor White EX 361-389 (BP) DM 415 (LP) EM 435 (LP)

PACKAGE CONTENT
• Supplied with power cord, dust cover and user manual
• Phase contrast models are supplied with green filter and alignment telescope

TECHNICAL SPECIFICATIONS
• Perfect for inexperienced users in need of training practice as well as for professionals
• Complete manual system
• Large movement range
• Easy micropipette position at each time ‘Manual Home Position’
• Adjustable micropipette holder angle between 20° and 40°
• Micropipette holder fixing knob reduces vibration and easy handling of the sperm
• Easy installation and service
• 3D Hydraulic Manipulation Joysticks for smooth movement
• Pneumatic Air Injector for ICSI and HOLDING
• No service required for injector
• Identical sensitive injectors on both sides and interchangeable 
• Top quality for affordable price
• Medical Device CE mark
• Movement Range: X/Y/Z: 20 mm each, 1 mm for one complete turn of the handle
• Optional heating stage with controller 

ISCI SET
Set includes two 3D hydraulic joysticks, two pipette setup mechanisms, one connection bar with moving handle, two air injectors with tubing and pen type micromanipulation, pipette holder, two metal plates for injectors, one set of Allen wrenches, one ruler for mechanic home position adjustment. Unit is packed in a solid carry case

HEATING STAGE
• Heating glass stage for Euromex microscope stage with 110 mm circular hole
• The glass plate fits the circular opening of the X-Y stage and enables a flat glass object-plate with constant temperature
• Aluminium frame for superior resistance and flatness 
• Glass thickness 0.5 mm. Plate diameter 110 mm

CONTROLLER
• 2-Channel Temperature Controller with touchscreen interface
• One controller can control up to two devices
• External temperature sensor (included) and self calibration routines allows easy calibration of the heating devices 
• On-board data logging allows to record temperature as a function of time and to log calibration events and alarms 
• The password protected controller is equipped with routines to re-calibrate the sensors against certified thermometers

FEATURES
•Temperature range: from ambient to 60°C
• Temperature accuracy on specimen: ± 0.3°C
• 4.3’’ touch screen interface
• External Temperature sensor
• On-board Data logging to USB drive
• Alarm buzzer and External Alarm Connector
• One controller can control up to two devices
• Self calibration routines enable high temperature accuracy